A Student's Guide to 
Patch-Clamp Electrophysiology
Pipette Puller
How to make whole-cell patch-clamp pipettes using the P97 Flaming/Brown pipette puller

Whole-Cell Patch Clamp Pipettes

Patch Pipette Program

💡 TIP: Earlier loops control the shape of the neck and later loops control the shape of the tip. To achieve improved control of tip properties you can copy the program values into four lines (loops) and modify the heat and velocity of only the last line.

How to Pull Pipettes

⚠️ WARNING: When pulling pipettes, it is EXTREMELY important to pay attention not to damage the filament inside the puller! Damaging the filament will prevent anyone in our laboratory from being able to pull pipettes or patch cells while a new puller is installed and calibrated. Therefore, if there is any uncertainty, do NOT proceed. Ask for help!