A Student's Guide to 
Patch-Clamp Electrophysiology
Aligning the Mira Laser
Notes related to power and alignment of a Coherent Mira Optima 900-F
Warning: This page may contain laboratory-specific information

Coherent Laser

Improving Mode Lock

Additional notes: I have found that I get better results if, once mode locked, I slowly decrease laser intensity (by adjusting the iris) back down to 200. As I do this, I often lose mode lock, so I re-establish mode lock, twiddle ALL the knobs to maximize the intensity of the mode-locked peak, then slowly narrow the iris again. After doing this 6-8 times, the laser will mode lock really easily (even though the original sine wave looks surprisingly small, and of the wrong frequency), and the mode-locked laser power will be a steady 200.

Photos and Diagrams

Adjustments

Left side

Right side

Not Locked

Mode Locked

Suggested Waveform

Ideal output spectrum and power

Micrometer Wavelength Adjustment

On 2018-10-19 the micrometer was adjusted to tune the laser to 810nm. This is the reading at optimal tuning position.